Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Assay time
Hands-on time
Input quantity
The AmpliSeq for Illumina Focus Panel is the recommended replacement for TruSight Tumor 26, which has been discontinued.
The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently.
The Focus Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Assay time | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Cancer type | Solid tumor |
| Content specifications | DNA and RNA targets for 52 oncogenes |
| Description | Somatic analysis research on 52 genes associated with solid tumor cancers. |
| Hands-on time | < 1.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System |
| Mechanism of action | Multiplex PCR |
| Method | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | DNA, RNA |
| Number of amplicons | 553 total. 2 pools. (DNA Pool: 269 amplicons. RNA Pool: 284 amplicons.) |
| Specialized sample types | FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) |
The AmpliSeq for Illumina Focus Panel panel includes both DNA and RNA workflows. Each workflow requires its own Library PLUS reaction and index adapter. RNA workflow also requires cDNA Synthesis.
AmpliSeq for Illumina Focus Panel
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| iSeq 100 System | 8 samples per run (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) |
2 × 150 bp |
| MiSeq System | Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×) |
2 × 150 bp |
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
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Pathology and clinical cancer research
Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.
| AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | AmpliSeq for Illumina Cancer Hotspot Panel v2 | TruSight Tumor 15 | |
|---|---|---|---|---|
| Assay time | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 7 hr |
| Cancer type | Solid tumor | Solid tumor | Pan-cancer, Solid tumor | Pan-cancer, Solid tumor |
| Content specifications | DNA and RNA targets for 52 oncogenes | DNA and RNA targets for 161 oncogenes | Hotspot regions of 50 genes with known associations to cancer | Amplifies 250 amplicons from 15 genes associated with solid tumors |
| Description | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. | NGS assay for 15 commonly mutated genes in solid tumors using 20 ng DNA. |
| Hands-on time | < 1.5 hr | <1.5 hr | < 1.5 hr | 3.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 20 ng FFPE DNA |
| Instruments | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode | MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System | MiSeq System, MiSeqDx in Research Mode, MiniSeq System |
| Mechanism of action | Multiplex PCR | Multiplex PCR | Multiplex PCR | |
| Method | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing |
| Multiplexing | 96 dual index combinations | 96 dual index combinations | 96 dual index combinations | 1-24-plex |
| Nucleic acid type | DNA, RNA | DNA, RNA | DNA | DNA |
| Number of amplicons | 553 total. 2 pools. (DNA Pool: 269 amplicons. RNA Pool: 284 amplicons.) | 4,648 total 4 pools (DNA pool 1: 1,891 amplicons. DNA pool 2: 1,890 amplicons. RNA pool 1: 447 amplicons. RNA pool 2: 420 amplicons.) | 207 total. 1 pool. | |
| Specialized sample types | FFPE tissue | FFPE tissue | FFPE tissue | FFPE tissue |
| Species category | Human | Human | Human | Human |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Somatic variants, Insertions-deletions (indels) | Somatic variants, Insertions-deletions (indels) |
High coverage uniformity and on-target alignment
DNA extracted from FFPE tissues of varying quality and HD samples was evaluated using the AmpliSeq for Illumina Focus Panel and sequenced on the MiniSeq and MiSeq Systems. Cq is an indicator of the quality of DNA islolated from FFPE tissues.
High concordance between expected and detected variant frequency
DNA from formalin-fixed HD samples was evaluated using the AmpliSeq for Illumina Focus Panel and sequenced on the MiniSeq and MiSeq Systems. Results showed 100% concordance between the expected detected variant frequency.
AmpliSeq™ Focus Panel for Illumina®
20019164
Focused DNA and RNA targeted panel that targets SNVs, Indels, CNVs and gene fusions. Kit contains 24 reactions. Library PLUS and index adapters sold separately.
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Ampliseq™ for Illumina® Focus Panel MiSeq Kit
20024183
The Ampliseq™ Focus Panel MiSeq Kit contains library prep and MiSeq sequencing reagents for 48 samples. This includes two AmpliSeq for Illumina® Focus Panels, one Ampliseq™ for Illumina® Library PLUS (96 Reactions), one Ampliseq™ for Illumina® CD Indexes Set A, and two MiSeq Reagent Kits v2 300 cycle.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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