Library Prep Automation
Automation Methods that Span Our Library Prep Portfolio
Minimize errors, reduce hands-on time, and enable higher throughput
Automate Your Library Prep
Automated liquid handling systems help laboratories obtain and maintain consistent results while driving efficiency. Automation also allows for the rapid scaling of throughput without the need for additional hands-on time.
Automation partners
Illumina has partnered with leading automation vendors to help provide automated methods for our library prep kits on their flagship systems. Combining their automation knowledge with our library prep and sequencing expertise helps create a valuable solution for NGS configured automated systems.
Illumina Qualified methods
Illumina Qualified methods are available for most of our library prep portfolio. These methods are developed, tested, distributed, owned, and supported by our partners. Illumina reviews data to ensure method performance and data quality.
Vendor developed methods
Vendor developed methods are those that have been developed by a partner, but have not undergone data review by Illumina. These methods are included on the chart below, but do not have the Illumina Qualified identifier.
Library Prep Automation Partner Chart
Please reference the chart and associated legend below to determine which methods are Illumina Qualified.
To view partner documentation, please click on the partner name at the top of the chart.
Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)
| Agilent | Beckman Coulter | Eppendorf | Hamilton | Revvity | Tecan | |
|---|---|---|---|---|---|---|
| Illumina Complete Long Read | Biomek i7 | epMotion 5075t | STAR MOA | Sciclone | ||
| Illumina Stranded Total RNA | Biomek i7 | epMotion 5075t | NGS STAR | Sciclone | DreamPrep® | |
| Illumina Stranded mRNA | Biomek i7 | epMotion 5075t | NGS STAR | Sciclone | DreamPrep | |
| Illumina RNA Prep with Enrichment | Bravo Option A | Biomek i7 NGeniuS |
epMotion 5075t | NGS STAR | Sciclone | DreamPrep |
| Illumina DNA PCR Free | Biomek i5 Biomek i7 NGeniuS |
epMotion 5075t | NGS STAR | Sciclone Zephyr |
DreamPrep | |
| Illumina DNA Prep | Bravo Option B Bravo Option A |
Biomek FXp Biomek iSeries NGeniuS |
epMotion 5075t | NGS STAR | Sciclone | DreamPrep Freedom EVO® NGS |
| Illumina DNA Prep with Enrichment | Bravo Option B Bravo Option A |
Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Sciclone | DreamPrep |
| Nextera XT DNA | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Sciclone | DreamPrep |
| AmpliSeq for Illumina* | Biomek i5 | epMotion 5075t epMotion 5073 |
NGS STAR NGS STARlet |
Sciclone | ||
| TruSight Oncology 500HT† | Biomek i7 | NGS STAR with 96 MPH/SBS STAR MOA | DreamPrep | |||
| TruSight Oncology 500 (DNA Only) | Biomek i7 NGeniuS |
NGS STAR MOA | ||||
| TruSight Oncology 500 | Biomek NXp Biomek i5 |
NGS STAR/SBS STAR LSA NGS STARlet |
Sciclone Fontus |
DreamPrep | ||
| TruSight Oncology 500 HRD | Biomek i7 | STAR MOA | DreamPrep | |||
| TruSight Tumor 15 | Biomek 4000 | epMotion 5075t | Zephyr | |||
| TruSight Tumor 170 | Biomek NXp | NGS STAR | ||||
| TruSeq DNA PCR-Free | Bravo Option B | Biomek FXp | epMotion 5075t | NGS STAR | Sciclone NGS and NGSx | Freedom EVO NGS DreamPrep |
| TruSeq Nano DNA | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Sciclone NGS and NGSx | Freedom EVO NGS |
| TruSeq Stranded mRNA | Bravo Option B Bravo Option A |
Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Sciclone NGS and NGSx | Freedom EVO NGS |
| TruSeq Stranded Total RNA | Bravo Option B | Biomek FXp | epMotion 5075t | NGS STAR | Sciclone NGS and NGSx | Freedom EVO NGS DreamPrep |
Starting in January 2022 Hamilton will refer to the NGS STAR configuration for TruSight Oncology library preparations as the SBS STAR LSA. All existing Illumina Qualified methods are backwards compatible and will continue to run on this system as this change is in name only.
*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.
† 500 High-throughput (HT)
Need additional information?
Contact our Automation Specialists to learn more about automation partners and Illumina Qualified methods.
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Automated Library Prep Workflow Examples
Assessing Nucleic Acid Quality with the Agilent Fragment Analyzer System
The Fragment Analyzer accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing. System options accommodate both low- and high-throughput labs.
DownloadAutomated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics
The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.
Download
Automating TruSight Oncology 500 High-Throughput
Learn more about automated methods from Beckman Coulter and Hamilton for streamlined, consistent library prep.
Read Hamilton Tech NoteRead Beckman Coulter Tech Note
Adopt High-Throughput Sequencing In Your Lab
High-throughput sequencing can cost-effectively run data-rich applications and process more samples to improve statistical signficance. Get rapid access to more data for greater insights.
Learn MoreLibrary Prep Technical Tips
DNA/RNA Isolation Considerations
Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.
dsDNA Library Concentration
Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.
Quantification and Quality Control
Find out how to quantify and validate final libraries for a successful sequencing run.