Liquid biopsy is most often associated with cell-free DNA, but new research has shown additional value in interrogating a broader diversity of analytes in the blood. Cell-free RNA (cfRNA) is one such molecule that has been shown to reflect dynamic changes in many tissues. Researchers at Illumina have optimized key variables to enable scalable, end-to-end workflow solutions for whole-transcriptome library preparations of cfRNA. Continued application of cfRNA stands to benefit a multitude of human health concerns by empowering non-invasive interrogations of disease etiology and identification of predictive, diagnostic, and prognostic biomarkers, all of which will further facilitate our transition into the era of personalized medicine. #liquidbiopsy #rna #rnaseq #sequencing #transcription Resources: Paper referred to in video - https://www.science.org/doi/10.1126/scitranslmed.aaz0131 RNA prep with Enrichment - https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/rna-prep-enrichment.html Presenters: Dan Gheba – Executive Sequencing Specialist, Illumina Suzanne Rohrback PhD – Senior Scientist, Advanced Sciences, Illumina 00:00 Introduction to Liquid Biopsy 01:25 Why RNA over DNA? 04:24 Illumina cfRNA Study Introduction 05:43 cfRNA quality and method development 08:52 Sample Collection best practices 10:50 Study findings – unique biomarkers 14:24 Machine learning model 16:52 Method Update! 19:09 Publication Summary 20:28 How can you use cfRNA in your research? Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM