Robust methylation profiling microarray providing extensive coverage of CpG islands, genes, and enhancers. Ideal for genetic and rare disease testing, cancer research and classification.
Sample throughput
Number of samples
Number of markers
The Infinium MethylationEPIC v2.0 kit is a genome-wide methylation analysis tool targeting ~930K unique methylation sites in the most biologically significant regions of the human methylome.
Genome-wide coverage at a relative affordable cost enables scalable discovery of powerful biological insights into disease mechanisms.
Illumina methylation BeadChips have powered > 10 years of groundbreaking research, with most published epigenome-wide association studies (EWAS) to date completed on MethylationEPIC or its predecessor. Harness methylation data in data repositories like the Gene Expression Omnibus (GEO) or The Cancer Genome Atlas (TCGA).
In addition to whole blood, Methylation EPICv2.0 is also compatible with FFPE sample types, enabling studies to be completed on large biorepositories of tumors.
| Assay type | Infinium HD Methylation |
|---|---|
| Automation capability | Automated array loader |
| Description | The Infinium MethylationEPIC v2.0 BeadChip is a genome-wide methylation analysis tool with enhanced, expert-selected functional content. The kit is processed on the iScan or NextSeq 550 Systems and follows a straightforward, user-friendly workflow that does not require sample pooling and indexing. |
| Input quantity | 250 ng DNA |
| Instruments | NextSeq 550 System, NextSeq 550Dx in Research Mode, iScan System |
| Method | Methylation array |
| Nucleic acid type | DNA |
| Number of markers | ~ 930k |
| Number of samples | 8 samples per array |
| Sample throughput | 3024 samples per week on a single iSCAN |
| Specialized sample types | Blood, FFPE tissue |
| Species category | Human |
| Technology | Microarray |
| Variant class | Differentially methylated cytosines |
Requires purchase of a kit that matches the number of samples you run at a time. Please note that each kit is processed as a single batch and is not designed to be divided across multiple experiments. If you are processing several low-throughput batches, order multiple small kits. The kits contain all required reagents for performing methylation screening, except for the bisulfite conversion kits which should be purchased separately from Zymo Research.
Infinium MethylationEPIC v2.0 BeadChip is a robust microarray that enables discovery of methylation-based biomarkers for research applications in cancer, genetic diseases, aging, and molecular epidemiology.
Infinium MethylationEPIC v2.0 Kit
Infinium HD Methylation Assay
Complex and genetic disease research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.
| Infinium MethylationEPIC v2.0 Kit | Infinium Custom Methylation Kit | Infinium Methylation Screening Array-48 Kit | Infinium Mouse Methylation BeadChip | |
|---|---|---|---|---|
| Assay type | Infinium HD Methylation | Infinium HD Methylation | ||
| Automation capability | Automated array loader | Automated array loader | Liquid handling robots, Automated array loader | Automated array loader |
| Description | The Infinium MethylationEPIC v2.0 BeadChip is a genome-wide methylation analysis tool with enhanced, expert-selected functional content. The kit is processed on the iScan or NextSeq 550 Systems and follows a straightforward, user-friendly workflow that does not require sample pooling and indexing. | Highly scalable and affordable methylation analysis of known common disease associations for population health applications. Ideal for studies >1,000 samples and up to millions of samples. | ||
| Input quantity | 250 ng DNA | 50 ng DNA | 250 ng DNA | |
| Instruments | NextSeq 550 System, NextSeq 550Dx in Research Mode, iScan System | iScan System | iScan System | iScan System |
| Method | Methylation array | Methylation array | Methylation array | Methylation array |
| Nucleic acid type | DNA | DNA | DNA | |
| Number of markers | ~ 930k | Fixed markers: 285k | ||
| Number of samples | 8 samples per array | 48 samples per array | 12 samples per array | |
| Sample throughput | 3024 samples per week on a single iSCAN | 16,128 samples per week | ||
| Specialized sample types | Blood, FFPE tissue | Blood, Cell-free DNA, Saliva | Low-input samples | Blood, FFPE tissue, Saliva |
| Species category | Human | Mouse, Human | Human | Mouse |
| Technology | Microarray | Microarray | Microarray | Microarray |
| Variant class | Differentially methylated cytosines | Differentially methylated cytosines | Differentially methylated cytosines | Differentially methylated cytosines |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
New and enhanced content enables future epigenetic discoveries.
CpG islands and exons that were covered insufficiently on the Infinium MethylationEPIC v1.0 BeadChip are covered thoroughly with additional probes.
FFPE tissue samples show robust performance with a modified version of the Infinium Methylation HD Assay. For precious samples and optimal sample integrity, the Infinium FFPE QC and DNA Restoration Kits are recommended.
The Infinium MethylationEPIC v2.0 BeadChip builds upon the existing CpG backbones of the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChips.
Inf MethylationEPIC V2.0 Kit (8 Spl)
20087706
Includes one, 8-sample BeadChip and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 8 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC V2.0 Kit (16 Spl)
20087707
Includes two, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 16 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC V2.0 Kit (32 Spl)
20087708
Includes four, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 32 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC V2.0 Kit (96 Spl)
20087709
Includes 12, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 96 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC-8+ v2.0 Kit (16 Spl)
20097750
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 2 beadchips with reagents for processing 16 samples.
Inf MethylationEPIC-8+ v2.0 Kit (32 Spl)
20097751
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 4 beadchips with reagents for processing 32 samples.
Inf MethylationEPIC-8+ v2.0 Kit (96 Spl)
20097752
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 12 beadchips with reagents for processing 96 samples.
Infinium® Assay: An Introduction – Customer Site
20015273
Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.
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The Infinium Methylation EPIC v2.0 BeadChip Kit leverages powerful Infinium chemistry to detect cytosine methylation at CpG islands based on highly multiplexed genotyping of bisulfite-converted genomic DNA. Learn more about quantitative array-based methylation measurement.
This kit uses the Illumina Methylation Assay, a microarray technology that provides quantitative methylation measurement at the single-CpG-site level, powering research that examines the biological role of DNA methylation in normal and diseased cells. Learn more about the Illumina Methylation Assay.
The Infinium MethylationEPIC v2.0 BeadChip Kit contains 186K new probes that target known enhancers, super-enhancers, CTCF-binding sites, and open regions of chromatin associated with primary tumors identified by ATAC-Seq and ChIP-seq experiments. Learn about additional improvements in the Infinium MethylationEPIC v2.0 data sheet.
MethylationEPICv2.0 data analysis is supported by Illumina Software. While GenomeStudio Software enables straightforward quality control and continues to be used by core laboratories for basic quality control, third party Bioconductor packages offer the most functionality for downstream analysis. Many free packages in the R software framework enable normalization and differential analysis of methylation data. See more methylation array data analysis tips.
The Zymo EZ DNA Methylation Lightning Kit is the recommended bisulfite conversion kit for the Infinium workflow. Third party bisulfite conversion kits must be purchased separately. Learn more about validated bisulfite conversion kits for Infinium Methylation BeadChips.
The scan takes ~18–37 minutes per BeadChip.
Yes, the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChip kits have been discontinued. The Infinium MethylationEPIC v2.0 Kit, built upon the same reliable Infinium chemistry foundation, offers enhanced, expert-selected content and is the recommended replacement for both kits.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Fully customizable methylation microarray for large, targeted epigenetic applications.
Features > 285k markers across the methylome for high-resolution epigenetic analyses of diverse murine strains.
Ultra-scalable methylation array optimized for population health research. Ideal for studies >1,000 samples, up to millions of samples.
Evaluate sample quality and repair degraded FFPE DNA samples for use in Infinium array-based assays.
A complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels.
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