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Discover the NextSeq 550 System
Learn how the NextSeq 550 System is enhancing research in reproductive and genetic health and why it’s being rapidly adopted in small and large genetics labs. Get a glimpse into the advantages of this integrated, cost-effective solution for diverse applications.
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An Illumina Field Applications Scientist discusses what to expect during the preinstallation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
Required products:
Qualification services for compliance with many regulations and quality standards
Optional products:
PhiX Control v3 for use as a sequencing control
Air filter replacement (recommended every 90 days)
NextSeq 550 Sequencing System
SY-415-1002
Illumina NextSeq 550 Sequencing System is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and array scanning. System includes embedded touchscreen monitor and on-instrument computer, NextSeq Control Software, installation and training, and 12 months warranty (including parts and labor).
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NextSeq 550 Installation and Operational Qualification
SP-104-5506
Verifies that the NextSeq 550 System has been delivered, installed, and is operating as intended according to manufacturer specifications. Appropriate documentation is provided.
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NextSeq 550 Performance Qualification
SP-104-5503
Verifies that the NextSeq 550 System is performing as intended according to manufacturer specifications. Appropriate documentation is provided.
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NextSeq 500 to NextSeq® 550 Upgrade
SY-415-1003
Upgrade NextSeq 500 to NextSeq 550 and enable array scanning of CytoSNP- 850k, CytoSNP-12, HumanKaryomap-12, Infinium MethylationEPIC v1.0, and Infinium MethylationEPIC v2.0 BeadChips. Upgrade cost will cover upgrade onsite by FSE.
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NextSeq Air Filter
20022240
Includes air filter to optimize performance by ensuring internal components remain free of dust and other environmental contaminants. Replace every 90 days as part of standard NextSeq recommended preventative maintenance.
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NextSeq PhiX Control Kit
FC-110-3002
DNA control for the NextSeq Illumina sequencing platform. Compatible with single and paired-end reads up to 150 bp. 10 µl of a 10 nM PhiX solution.
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Run duration depends on the type of flow cell and number of cycles. For more information and sample run times, see the NextSeq 550 System data sheet in the product literature section of this page.
Scanning takes approximately 40 minutes per BeadChip. The Infinium CytoSNP-850K and Infinium MethylationEPIC v2.0 BeadChips can be scanned at a rate of approximately 5 minutes per sample. The HumanCytoSNP-12 and HumanKaryomap-12 BeadChips are scanned at a rate of 3.5 minutes per sample.
No, simultaneous sequencing and array scanning is not possible on the NextSeq 550 System because the BeadChip and flow cell use the same imaging components.
Yes, NextSeq 500/550 kits include reagents for paired-end sequencing and a paired-end flow cell. However, single-read runs are possible on a paired-end flow cell when the run is set up as a single-read run.
The data set generated by the NextSeq 550 System is too large for on-instrument analysis. Data must be transferred to BaseSpace Sequence Hub or a local server for secondary analysis.
NextSeq 550 Systems running NCS v4 or later and use Local Run Manager software modules for on-instrument analysis. Additional analysis tools are available on BaseSpace Sequence Hub. You can also configure the NextSeq 550 System to transfer data to a local server and perform analysis using third-party software.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Use these components in ATAC-Seq experiments to analyze chromatin accessibility.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
A fast, flexible, and scalable cfDNA library preparation kit for highly sensitive mutation detection
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Library prep and enrichment kit that delivers highly sensitive, comprehensive respiratory pathogen identification and antimicrobial resistance insights.
Verify instrument performance and obtain an audit-ready PQ report from certified Illumina engineers to help meet regulatory requirements.
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